【Abstract】 Objective To study the method to identify Brucella suis biovar 1 wild strain and S2 vaccine strain.  Methods A total of 21 strains of B.suis biovar 1 wild strain were analyzed by AMOS-PCR, PFGE and multiple-locus variable-number tandem-repeat analysis (MLVA).   Results B.suis biovar 1 and S2 could be discriminated by MLVA Bru9 and Bru16. Conclusion The MLVA assay can be applied to identification of wild strains and vaccine strain and can be proposed to as a complement of classical biotyping methods.